fkbp51 goat polyclonal antibody  (R&D Systems)

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Greater in situ FKBP51 protein and mRNA levels in leiomyoma vs. paired myometrial tissues. ( A ) FKBP51 (brown) and alpha-smooth muscle actin (red) double immunostaining in paired myometrium and adjacent leiomyoma sections. Menstrual cycle phases were proliferative phase (PRO, cycle day 10–14) or secretory phase (SEC, cycle day 22–26). MM: myometrium, LM: uterine leiomyoma. Bars represent Mean ± SEM. *P < 0.001 vs . MM; Ϯ P = 0.047 vs . MM; # P = 0.015 vs . MM. Inset image shows negative staining as a control. Original magnification 40x. ( B ) FKBP51 and β actin protein levels detected by immunoblotting in MM and LM tissues. Results were quantified using ImageJ and normalized to β actin. Control is decidual cell lysates without (c) and with dexamethasone treatment (c + Dex). P1, P2 are proliferative phase samples, and S1, S2 are secretory phase samples. Bars represent Mean ± SEM. *P < 0.001 vs . MM; Ϯ P = 0.003 vs . MM. ( C ) FKBP5 mRNA levels in LM compared to MM tissues detected by qPCR. Bars represent Mean ± SEM; *P < 0.001 vs . MM; Ϯ P = 0.013 vs . MM; # P = 0.034 vs . MM

Article Snippet: The membranes were then incubated overnight at 4 0 C with a goat polyclonal antibody for FKBP51 (R&D AF4094) in TBS-T containing 5% BSA, followed by incubation with horseradish peroxidase conjugated rabbit anti-goat (VECTOR PI-9500).

Techniques: In Situ, Double Immunostaining, Negative Staining, Control, Western Blot

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Silencing FKBP5 in cultured leiomyoma cells downregulates cell survival factors. Analyses of FKBP51 mRNA ( A ) and protein ( B ) levels by qPCR and immunoblotting, receptively, in leiomyoma cell cultures at 72 h after transfection with FKBP5 siRNA. Expression levels of PCNA, MKI67, CCND1, BCL2, BAX and BAD mRNA ( C ) and PCNA protein ( D ) levels in leiomyoma cell cultures at 72 h of transfection with control (scramble) or FKBP5 siRNA. Bars represent Mean ± SEM. *P < 0.001 vs. corresponding control siRNA; Ϯ P < 0.050 vs. corresponding control siRNA

Article Snippet: The membranes were then incubated overnight at 4 0 C with a goat polyclonal antibody for FKBP51 (R&D AF4094) in TBS-T containing 5% BSA, followed by incubation with horseradish peroxidase conjugated rabbit anti-goat (VECTOR PI-9500).

Techniques: Cell Culture, Western Blot, Transfection, Expressing, Control

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Reduced BrdU incorporation in leiomyoma cell cultures after transfection with FKBP5 siRNA. ( A ) Levels of BrdU incorporation in control (C), estradiol (E); estradiol + medroxyprogesterone (EM)-treated leiomyoma cell cultures at 72 h of transfection with control- (scramble) or FKBP5 siRNA. Bars represent Mean ± SEM. *P < 0.001 vs. corresponding control siRNA; # P < 0.05 vs. control FKBP5 siRNA; Ϯ P \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\le$$\end{document} ≤ 0.005 vs. non-hormonal control siRNA; n = 3. ( B ) BrdU incorporation after treating cells with epidermal growth factor (EGF) both in leiomyoma cell cultures at 72 h of transfection with control or FKBP5 siRNA. Bars represent Mean ± SEM. *P < 0.050 vs. EM control siRNA; n = 3

Article Snippet: The membranes were then incubated overnight at 4 0 C with a goat polyclonal antibody for FKBP51 (R&D AF4094) in TBS-T containing 5% BSA, followed by incubation with horseradish peroxidase conjugated rabbit anti-goat (VECTOR PI-9500).

Techniques: BrdU Incorporation Assay, Transfection, Control

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Apoptotic index in leiomyoma cell cultures transfection with control or FKBP5 siRNA. Levels of apoptosis in control (C) or estradiol (E) or estradiol + medroxyprogesterone (EM)-treated leiomyoma cell cultures following transfection with control or FKBP5 siRNA for 72 h. Bars represent Mean ± SEM; n = 3

Article Snippet: The membranes were then incubated overnight at 4 0 C with a goat polyclonal antibody for FKBP51 (R&D AF4094) in TBS-T containing 5% BSA, followed by incubation with horseradish peroxidase conjugated rabbit anti-goat (VECTOR PI-9500).

Techniques: Transfection, Control

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: FKBP5 silencing decreased mRNA and protein levels of ECM proteins and TIMPs in leiomyoma cells. ( A ) Expression levels of collagen type 1 alpha 1 chain ( COL1A1 ), collagen type IV alpha 5 chain ( COL4A5 ), collagen type VII alpha 1 chain ( COL7A1 ), collagen type X alpha 1 chain ( COL10A1 ), fibronectin 1 ( FN1 ); Laminin Subunit Gamma 1 ( LAMC1 ) mRNAs in leiomyoma cells after treatment with control siRNA or FKBP5 siRNA for 72 h. ( B ) Tissue inhibitor of metalloproteinases TIMP1 and TIMP3 and matrix metalloproteinase 1 ( MMP1 ) levels in control or FKBP5 siRNA-treated leiomyoma cell cultures for 72 h. Protein levels for LAMC1 and TIMP1 ( C ) detected by immunoblotting in control or FKBP5 siRNA-treated leiomyoma cell cultures for 72 h were also compared. Bars represent Mean ± SEM; n = 4. *P < 0.010 vs. corresponding control. ** P < 0.050 vs. corresponding control siRNA

Article Snippet: The membranes were then incubated overnight at 4 0 C with a goat polyclonal antibody for FKBP51 (R&D AF4094) in TBS-T containing 5% BSA, followed by incubation with horseradish peroxidase conjugated rabbit anti-goat (VECTOR PI-9500).

Techniques: Expressing, Control, Western Blot

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Greater in situ FKBP51 protein and mRNA levels in leiomyoma vs. paired myometrial tissues. ( A ) FKBP51 (brown) and alpha-smooth muscle actin (red) double immunostaining in paired myometrium and adjacent leiomyoma sections. Menstrual cycle phases were proliferative phase (PRO, cycle day 10–14) or secretory phase (SEC, cycle day 22–26). MM: myometrium, LM: uterine leiomyoma. Bars represent Mean ± SEM. *P < 0.001 vs . MM; Ϯ P = 0.047 vs . MM; # P = 0.015 vs . MM. Inset image shows negative staining as a control. Original magnification 40x. ( B ) FKBP51 and β actin protein levels detected by immunoblotting in MM and LM tissues. Results were quantified using ImageJ and normalized to β actin. Control is decidual cell lysates without (c) and with dexamethasone treatment (c + Dex). P1, P2 are proliferative phase samples, and S1, S2 are secretory phase samples. Bars represent Mean ± SEM. *P < 0.001 vs . MM; Ϯ P = 0.003 vs . MM. ( C ) FKBP5 mRNA levels in LM compared to MM tissues detected by qPCR. Bars represent Mean ± SEM; *P < 0.001 vs . MM; Ϯ P = 0.013 vs . MM; # P = 0.034 vs . MM

Article Snippet: Slides were treated with 3% hydrogen peroxidase to block endogenous peroxidase activity for 20 min and then followed by antigen retrieval by boiling in citrate buffer solution (10 mmol/L; pH 6.0) for 30 min. After rinsing in Tris-buffered saline (TBS), slides were incubated with 10% normal horse serum (Vector Labs, Burlingame, CA) in a humidified chamber for 30 min at room temperature (RT) and then incubated at 4 0 C overnight with goat polyclonal antibody against FKBP51 (1:3000, R&D Minneapolis, MN).

Techniques: In Situ, Double Immunostaining, Negative Staining, Control, Western Blot

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Silencing FKBP5 in cultured leiomyoma cells downregulates cell survival factors. Analyses of FKBP51 mRNA ( A ) and protein ( B ) levels by qPCR and immunoblotting, receptively, in leiomyoma cell cultures at 72 h after transfection with FKBP5 siRNA. Expression levels of PCNA, MKI67, CCND1, BCL2, BAX and BAD mRNA ( C ) and PCNA protein ( D ) levels in leiomyoma cell cultures at 72 h of transfection with control (scramble) or FKBP5 siRNA. Bars represent Mean ± SEM. *P < 0.001 vs. corresponding control siRNA; Ϯ P < 0.050 vs. corresponding control siRNA

Article Snippet: Slides were treated with 3% hydrogen peroxidase to block endogenous peroxidase activity for 20 min and then followed by antigen retrieval by boiling in citrate buffer solution (10 mmol/L; pH 6.0) for 30 min. After rinsing in Tris-buffered saline (TBS), slides were incubated with 10% normal horse serum (Vector Labs, Burlingame, CA) in a humidified chamber for 30 min at room temperature (RT) and then incubated at 4 0 C overnight with goat polyclonal antibody against FKBP51 (1:3000, R&D Minneapolis, MN).

Techniques: Cell Culture, Western Blot, Transfection, Expressing, Control

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Reduced BrdU incorporation in leiomyoma cell cultures after transfection with FKBP5 siRNA. ( A ) Levels of BrdU incorporation in control (C), estradiol (E); estradiol + medroxyprogesterone (EM)-treated leiomyoma cell cultures at 72 h of transfection with control- (scramble) or FKBP5 siRNA. Bars represent Mean ± SEM. *P < 0.001 vs. corresponding control siRNA; # P < 0.05 vs. control FKBP5 siRNA; Ϯ P \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\le$$\end{document} ≤ 0.005 vs. non-hormonal control siRNA; n = 3. ( B ) BrdU incorporation after treating cells with epidermal growth factor (EGF) both in leiomyoma cell cultures at 72 h of transfection with control or FKBP5 siRNA. Bars represent Mean ± SEM. *P < 0.050 vs. EM control siRNA; n = 3

Article Snippet: Slides were treated with 3% hydrogen peroxidase to block endogenous peroxidase activity for 20 min and then followed by antigen retrieval by boiling in citrate buffer solution (10 mmol/L; pH 6.0) for 30 min. After rinsing in Tris-buffered saline (TBS), slides were incubated with 10% normal horse serum (Vector Labs, Burlingame, CA) in a humidified chamber for 30 min at room temperature (RT) and then incubated at 4 0 C overnight with goat polyclonal antibody against FKBP51 (1:3000, R&D Minneapolis, MN).

Techniques: BrdU Incorporation Assay, Transfection, Control

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: Apoptotic index in leiomyoma cell cultures transfection with control or FKBP5 siRNA. Levels of apoptosis in control (C) or estradiol (E) or estradiol + medroxyprogesterone (EM)-treated leiomyoma cell cultures following transfection with control or FKBP5 siRNA for 72 h. Bars represent Mean ± SEM; n = 3

Article Snippet: Slides were treated with 3% hydrogen peroxidase to block endogenous peroxidase activity for 20 min and then followed by antigen retrieval by boiling in citrate buffer solution (10 mmol/L; pH 6.0) for 30 min. After rinsing in Tris-buffered saline (TBS), slides were incubated with 10% normal horse serum (Vector Labs, Burlingame, CA) in a humidified chamber for 30 min at room temperature (RT) and then incubated at 4 0 C overnight with goat polyclonal antibody against FKBP51 (1:3000, R&D Minneapolis, MN).

Techniques: Transfection, Control

Journal: Reproductive Sciences

Article Title: FKBP51 Contributes to Uterine Leiomyoma Pathogenesis by Inducing Cell Proliferation and Extracellular Matrix Deposition

doi: 10.1007/s43032-022-00921-2

Figure Lengend Snippet: FKBP5 silencing decreased mRNA and protein levels of ECM proteins and TIMPs in leiomyoma cells. ( A ) Expression levels of collagen type 1 alpha 1 chain ( COL1A1 ), collagen type IV alpha 5 chain ( COL4A5 ), collagen type VII alpha 1 chain ( COL7A1 ), collagen type X alpha 1 chain ( COL10A1 ), fibronectin 1 ( FN1 ); Laminin Subunit Gamma 1 ( LAMC1 ) mRNAs in leiomyoma cells after treatment with control siRNA or FKBP5 siRNA for 72 h. ( B ) Tissue inhibitor of metalloproteinases TIMP1 and TIMP3 and matrix metalloproteinase 1 ( MMP1 ) levels in control or FKBP5 siRNA-treated leiomyoma cell cultures for 72 h. Protein levels for LAMC1 and TIMP1 ( C ) detected by immunoblotting in control or FKBP5 siRNA-treated leiomyoma cell cultures for 72 h were also compared. Bars represent Mean ± SEM; n = 4. *P < 0.010 vs. corresponding control. ** P < 0.050 vs. corresponding control siRNA

Article Snippet: Slides were treated with 3% hydrogen peroxidase to block endogenous peroxidase activity for 20 min and then followed by antigen retrieval by boiling in citrate buffer solution (10 mmol/L; pH 6.0) for 30 min. After rinsing in Tris-buffered saline (TBS), slides were incubated with 10% normal horse serum (Vector Labs, Burlingame, CA) in a humidified chamber for 30 min at room temperature (RT) and then incubated at 4 0 C overnight with goat polyclonal antibody against FKBP51 (1:3000, R&D Minneapolis, MN).

Techniques: Expressing, Control, Western Blot

Journal: PLoS ONE

Article Title: Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding

doi: 10.1371/journal.pone.0137855

Figure Lengend Snippet: 40 most highly differentially regulated genes in HESCs treated by E 2 +MPA vs . E 2 alone according to whole genome microarray analysis using Illumina HumanHT–12 v4 expression BeadChip kit. All gene symbols were abbreviated according to GENEBANK standard nomenclature.

Article Snippet: After transfer, the membranes were blocked overnight in Tris-Buffered Saline (TBS) with 10% non-fat dry milk and then incubated with goat anti-FKBP51 polyclonal antibody at 1/1000 dilution (R&D Systems) or with rabbit anti-total (T) and phosphorylated (P)-AKT, or with rabbit anti-T and P-ERK1/2 MAPK antibodies (Cell Signaling) at 1/1000 dilution for primary antibody labeling.

Techniques: Microarray, Expressing, Clinical Proteomics, Membrane

Journal: PLoS ONE

Article Title: Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding

doi: 10.1371/journal.pone.0137855

Figure Lengend Snippet: (A) q-PCR and (B) immunoblot analysis of FKBP51 levels in HESCs treated with estradiol (E 2 , 10 −8 M) ± progesterone (P 4 , 10 −7 M) or etonogestrel (ETO, 10 −7 M) or medroxyprogesterone acetate (MPA, 10 −7 M) for 6h or 24 h, respectively. Bars represent mean ± SEM (n = 3). * p<0 . 001 in E 2 +ETO vs . E 2 ; # p<0 . 001 in E 2 +MPA vs . E 2 .

Article Snippet: After transfer, the membranes were blocked overnight in Tris-Buffered Saline (TBS) with 10% non-fat dry milk and then incubated with goat anti-FKBP51 polyclonal antibody at 1/1000 dilution (R&D Systems) or with rabbit anti-total (T) and phosphorylated (P)-AKT, or with rabbit anti-T and P-ERK1/2 MAPK antibodies (Cell Signaling) at 1/1000 dilution for primary antibody labeling.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding

doi: 10.1371/journal.pone.0137855

Figure Lengend Snippet: (A_C) FKBP51 expression in paired endometrium of women pre- and post- DMPA administration. Immunoreactive FKBP51 in endometrial stromal (arrowheads) and glandular (arrow) cells pre- (A) and post- (B) DMPA use. HSCORE analysis of FKBP51 expression (C) in stromal and glandular cells. Bars represent mean ± SEM (n = 6). * p< 0 . 01 and # p<0 . 01 . Pre-DMPA: before DMPA use; Post-DMPA: 3 months DMPA use. (D-H) FKBP51 expression in endometria of OVX-GPs treated by MPA. Immunoreactivity for FKBP51 (brown) in stromal (arrowhead) and epithelial (arrows) cells of endometria of OVX-GPs treated with vehicle (D) or E 2 (E) or MPA (F) or E 2 +MPA (G) for 21 days. HSCORE analysis of FKBP51 immunoreactivity (H) in stromal and glandular cells. Bars represent mean ± SEM (n = 3 per treatment group). † p<0 . 001 in E 2 +MPA vs . E 2 or placebo (Pla); ѱ p<0 . 001 in E 2 +MPA vs . MPA or E 2 or Pla; ¥ in p<0 . 05 in MPA vs . E 2 or Pla; ‡ p<0 . 05 in MPA vs . E 2 and Pla. Original Magnification: A-G x40.

Article Snippet: After transfer, the membranes were blocked overnight in Tris-Buffered Saline (TBS) with 10% non-fat dry milk and then incubated with goat anti-FKBP51 polyclonal antibody at 1/1000 dilution (R&D Systems) or with rabbit anti-total (T) and phosphorylated (P)-AKT, or with rabbit anti-T and P-ERK1/2 MAPK antibodies (Cell Signaling) at 1/1000 dilution for primary antibody labeling.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding

doi: 10.1371/journal.pone.0137855

Figure Lengend Snippet: (A) Basal IL-1β mRNA levels in 10 −8 M estradiol (E) vs . E+ 10 −7 M MPA (EM) treated HESCs at 44 hrs post-transfection with either a control (Cont-V) or FKBP51 vector (FKBP51-V). Bars represent mean ± SEM (n = 4 with 3 replicates per treatment group). (B) In parallel incubations, thrombin- (Th-) induced IL-1β mRNA levels in E 2 +MPA treated HESCs at 44 hrs post-transfection with control or FKBP51 vector. Bars represent mean ± SEM (n = 2 and 3 replicates per treatment group). E: E 2 and EM: E 2 +MPA, * p<0 . 05 vs . EM treated in Cont-V transfected HESCs; ¥ p<0 . 05 vs . EM treatment in Cont-V transfected HESCs; † p<0 . 05 vs . EM; ‡ p<0 . 05 vs . EM+Th.

Article Snippet: After transfer, the membranes were blocked overnight in Tris-Buffered Saline (TBS) with 10% non-fat dry milk and then incubated with goat anti-FKBP51 polyclonal antibody at 1/1000 dilution (R&D Systems) or with rabbit anti-total (T) and phosphorylated (P)-AKT, or with rabbit anti-T and P-ERK1/2 MAPK antibodies (Cell Signaling) at 1/1000 dilution for primary antibody labeling.

Techniques: Transfection, Control, Plasmid Preparation

Journal: PLoS ONE

Article Title: Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding

doi: 10.1371/journal.pone.0137855

Figure Lengend Snippet: LAPCs reduce endometrial blood flow causing hypoxia (HX) and generating reactive oxygen species (ROS), to directly damage blood vessels and enhance HEEC expression of angiopoietin (Ang)-2 and HESC expression of vascular endothelial growth factor (VEGF), interleukin (IL)-8 and matrix metalloproteinases (MMPs) while inhibiting HESC Ang–1 expression. The resultant inflammation and aberrant angiogenic stimulus leads to vessel damage causing local bleeding. The later delivers circulating factor VII to HESC membrane bound tissue factor (TF) which activates factor Xa to ultimately generate thrombin. The later exacerbates HX/ROS effects on endometrial angiogenesis and inflammation. In turn, HX and LAPCs trigger HEEC apoptosis by a paracrine manner. In addition, LAPCs also enhance FKBP51 expression in HESCs to inhibit progesterone receptor (PR; NR3C3) and glucocorticoid receptor (GR; NR3C1)-mediated transcription. In turn, the resultant PR and/or GR-mediated functional withdrawal reduces progestin and glucocorticoid mediated inhibition of endometrial inflammation, aberrant angiogenesis, thus contributing to AUB.

Article Snippet: After transfer, the membranes were blocked overnight in Tris-Buffered Saline (TBS) with 10% non-fat dry milk and then incubated with goat anti-FKBP51 polyclonal antibody at 1/1000 dilution (R&D Systems) or with rabbit anti-total (T) and phosphorylated (P)-AKT, or with rabbit anti-T and P-ERK1/2 MAPK antibodies (Cell Signaling) at 1/1000 dilution for primary antibody labeling.

Techniques: Expressing, Membrane, Functional Assay, Inhibition

Journal: PLoS ONE

Article Title: Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding

doi: 10.1371/journal.pone.0137855

Figure Lengend Snippet: 40 most highly differentially regulated genes in HESCs treated by E 2 +ETO vs . E 2 alone according to whole genome microarray analysis using Illumina HumanHT–12 v4 expression BeadChip kit. All gene symbols were abbreviated according to GENEBANK standard nomenclature.

Article Snippet: After transfer, the membranes were blocked overnight in Tris-Buffered Saline (TBS) with 10% non-fat dry milk and then incubated with goat anti-FKBP51 polyclonal antibody at 1/1000 dilution (R&D Systems) or with rabbit anti-total (T) and phosphorylated (P)-AKT, or with rabbit anti-T and P-ERK1/2 MAPK antibodies (Cell Signaling) at 1/1000 dilution for primary antibody labeling.

Techniques: Microarray, Expressing, Clinical Proteomics, Membrane